https://www.sciencedaily.com/releases/2019/04/190410125617.htm

Researchers at Purdue University have created an electron microscopy technique termed "cryoAPEX" that accurately tracks membrane proteins in a well-preserved cell. The new method is a hybrid of two commonly used methods in cell biology: high-pressure freezing and chemical fixation.

"We took the best features from each technique and played around with conditions until we found a happy medium where you could stain for your protein while maintaining membrane preservation," said Seema Mattoo, an assistant professor of biological sciences at Purdue. "We were also able to use this information to develop a 3D image of the protein in the context of the cell."