https://phys.org/news/2018-06-dynamics-thousands-protein-complexes-simultaneously.html

Methods to capture snapshots of protein-protein interaction networks in cells have been described before, but as Tan explains, "until now there has been no way of monitoring the dynamics of these protein complexes in a high-throughput and untargeted manner".

Exposing proteins to increasing temperature causes them to precipitate out of solution. Thermal proximity coaggregation (TPCA) is based on the idea that proteins that are part of a stable protein complex will coprecipitate, by virtue of close proximity, and have a similar precipitation profile across different temperatures (or melting curve).